Patent pending „Polynucleotides for enhancing expression of a polynucleotide of interest“
In cooperation with
the University of Applied Sciences Mannheim (Institut für Molekular-
und Zellbiologie) we developed a new expression system
enabling the fast and easy generating of high producer
cell clones. After integration into the host chromatin special
stabilizing and enhancing sequences leads to prevention of gene.
resulting in stable cell
lines with increased recombinant protein expression and a
better long term stability of expression.
Our new technology enables the fast and easy generation of high
producer cell lines.
Contract MAB Production
Optimal conditions for cell
growth and MAB production are determined on shaker flask level.
Appropriate media for the promotion of optimal growth and the best
operating parameters for maximum MAB production are determined.
Operation of the bioreactor is
closely monitored to ensure adherence to defined production
parameters. Cell growth and antibody production are assessed
with the periodic measurement of glucose uptake, pH, lactate
concentration, antibody yield, temperature, gas exchange, etc.
Contract MAB Purification
With the protein A/G method a mixed subclass IgG preparation
can be separated out by eluting a protein A/G column in a single step.
This method is highly specific and can be used with almost all isotypes of IgG’s.
The purified antibody is then
bottled and stored in aliquots in liquid, frozen or lyophilized form
depending on the product specifications. Q.C. analysis
assessing the purified antibody concentration, purity and functionality is